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Identification and characterization of chalcone synthase cDNAs (NnCHS) from Nelumbo nucifera.
Dong, C; Yu, A Q; Wang, M L; Zheng, X W; Diao, Y; Xie, K Q; Zhou, M Q; Hu, Z L.
Afiliación
  • Dong C; Henan University of Technology College of Biological Engineering Zhengzhou China.
  • Yu AQ; Wuhan University State Key Laboratory of Hybrid Rice, College of Life Science Wuhan China.
  • Wang ML; Wuhan University State Key Laboratory of Hybrid Rice, College of Life Science Wuhan China.
  • Zheng XW; Guangchang White Lotus Research Institute Fuzhou China.
  • Diao Y; Wuhan University State Key Laboratory of Hybrid Rice, College of Life Science Wuhan China.
  • Xie KQ; Guangchang White Lotus Research Institute Fuzhou China.
  • Zhou MQ; Wuhan University Lotus Center Wuhan China.
  • Hu ZL; Wuhan University State Key Laboratory of Hybrid Rice, College of Life Science Wuhan China huzhongli@whu.edu.cn.
Cell Mol Biol (Noisy-le-grand) ; 61(8): 112-7, 2015 Dec 26.
Article en En | MEDLINE | ID: mdl-26718438
Chalcone synthase (CHS) catalyzes the first committed step in flavonoids biosynthetic pathway. In this study, six full-length cDNAs (NnCHS) encoding CHS from Nelumbo nucifera were successfully isolated, using rapid amplification cDNA end (RACE) assay. The obtained cDNAs were 1426 bp in size, containing a 1167 bp open reading frame coding 389 amino acids. Exons-intron architecture of NnCHS gene was illustrated, consisting two exons inserted by a 426 bp intron. The putative NnCHS possessed all the conserved active sites for CHS function as well as the family signature. Phylogenetic analysis revealed that NnCHS shared high homology with CHS from high plants, and the homology-based structural modeling showed that NnCHS had the typical structure of CHS. Moreover, Real-time PCR assays demonstrated that NnCHS mRNAs were expressed in various tissues of N. nucifera, with the highest expression in red flower and lowest level in the leaves. Moreover, patterns of NnCHS expression illustrated short-time wounding or low temperature significantly induced the up-regulation of NnCHS mRNA.
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Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Aciltransferasas / Hojas de la Planta / Regulación de la Expresión Génica de las Plantas / Nelumbo / Flores Tipo de estudio: Diagnostic_studies Idioma: En Revista: Cell Mol Biol (Noisy-le-grand) Asunto de la revista: BIOLOGIA MOLECULAR Año: 2015 Tipo del documento: Article
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Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Aciltransferasas / Hojas de la Planta / Regulación de la Expresión Génica de las Plantas / Nelumbo / Flores Tipo de estudio: Diagnostic_studies Idioma: En Revista: Cell Mol Biol (Noisy-le-grand) Asunto de la revista: BIOLOGIA MOLECULAR Año: 2015 Tipo del documento: Article