Identification of a novel mitochondrial interacting protein of C1QBP using subcellular fractionation coupled with CoIP-MS.

Chen, Ruibing; Xiao, Mingming; Gao, Huajun; Chen, Yajing; Li, Yongmei; Liu, Yunde; Zhang, Ning

Chen, Ruibing; Xiao, Mingming; Gao, Huajun; Chen, Yajing; Li, Yongmei; Liu, Yunde; Zhang, Ning.

Afiliación

Chen R; Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy; Research Center of Basic Medical Sciences; School of Medical Laboratory, Tianjin Medical University, Tianjin, 300070, China. chenruibing@tijmu.edu.
Xiao M; Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy; Research Center of Basic Medical Sciences; School of Medical Laboratory, Tianjin Medical University, Tianjin, 300070, China.
Gao H; Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy; Research Center of Basic Medical Sciences; School of Medical Laboratory, Tianjin Medical University, Tianjin, 300070, China.
Chen Y; Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy; Research Center of Basic Medical Sciences; School of Medical Laboratory, Tianjin Medical University, Tianjin, 300070, China.
Li Y; Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy; Research Center of Basic Medical Sciences; School of Medical Laboratory, Tianjin Medical University, Tianjin, 300070, China.
Liu Y; Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy; Research Center of Basic Medical Sciences; School of Medical Laboratory, Tianjin Medical University, Tianjin, 300070, China.
Zhang N; Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy; Research Center of Basic Medical Sciences; School of Medical Laboratory, Tianjin Medical University, Tianjin, 300070, China. zhangning@tijmu.edu.cn

Anal Bioanal Chem ; 408(6): 1557-64, 2016 Feb.

Article en En | MEDLINE | ID: mdl-26753982

Asunto(s)

Autoantígenos/metabolismo; Proteínas Portadoras/metabolismo; Acetiltransferasa de Residuos Dihidrolipoil-Lisina/metabolismo; Inmunoprecipitación/métodos; Proteínas Mitocondriales/análisis; Espectrometría de Masas en Tándem/métodos; Autoantígenos/análisis; Proteínas Portadoras/análisis; Proteínas Portadoras/genética; Fraccionamiento Químico/métodos; Acetiltransferasa de Residuos Dihidrolipoil-Lisina/análisis; Células HEK293; Humanos; Mitocondrias/química; Mitocondrias/metabolismo; Proteínas Mitocondriales/genética; Proteínas Mitocondriales/aislamiento & purificación; Proteínas Mitocondriales/metabolismo; Mapeo de Interacción de Proteínas/métodos; Complejo Piruvato Deshidrogenasa/metabolismo; Reproducibilidad de los Resultados

Palabras clave

C1QBP; CoIP-MS/MS; LC-MS/MS; Mitochondria; Protein-protein interaction; Subcellular fractionation

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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Autoantígenos / Proteínas Portadoras / Proteínas Mitocondriales / Inmunoprecipitación / Acetiltransferasa de Residuos Dihidrolipoil-Lisina / Espectrometría de Masas en Tándem Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Anal Bioanal Chem Año: 2016 Tipo del documento: Article

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