A microfluidic array for real-time live-cell imaging of human and rodent pancreatic islets.
Lab Chip
; 16(8): 1466-72, 2016 Apr 21.
Article
en En
| MEDLINE
| ID: mdl-26999734
ABSTRACT
In this study, we present a microfluidic array for high-resolution imaging of individual pancreatic islets. The device is based on hydrodynamic trapping principle and enables real-time analysis of islet cellular responses to insulin secretagogues. This device has significant advantages over our previously published perifusion chamber device including significantly increased analytical power and assay sensitivity, as well as improved spatiotemporal resolution. The islet array, with live-cell multiparametric imaging integration, provides a better tool to understand the physiological and pathophysiological changes of pancreatic islets through the analysis of single islet responses. This platform demonstrates the feasibility of array-based islet cellular analysis and opens up a new modality to conduct informative and quantitive evaluation of islets and cell-based screening for new diabetes treatments.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Islotes Pancreáticos
/
Imagen Molecular
/
Dispositivos Laboratorio en un Chip
Idioma:
En
Revista:
Lab Chip
Asunto de la revista:
BIOTECNOLOGIA
/
QUIMICA
Año:
2016
Tipo del documento:
Article