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Extracellular purines' action on glomerular albumin permeability in isolated rat glomeruli: insights into the pathogenesis of albuminuria.
Kasztan, Malgorzata; Piwkowska, Agnieszka; Kreft, Ewelina; Rogacka, Dorota; Audzeyenka, Irena; Szczepanska-Konkel, Miroslawa; Jankowski, Maciej.
Afiliación
  • Kasztan M; Department of Therapy Monitoring and Pharmacogenetics, Medical University of Gdansk, Gdansk, Poland;
  • Piwkowska A; Laboratory of Molecular and Cellular Nephrology, Mossakowski Medical Research Centre Polish Academy of Sciences, Gdansk, Poland.
  • Kreft E; Department of Therapy Monitoring and Pharmacogenetics, Medical University of Gdansk, Gdansk, Poland;
  • Rogacka D; Laboratory of Molecular and Cellular Nephrology, Mossakowski Medical Research Centre Polish Academy of Sciences, Gdansk, Poland.
  • Audzeyenka I; Laboratory of Molecular and Cellular Nephrology, Mossakowski Medical Research Centre Polish Academy of Sciences, Gdansk, Poland.
  • Szczepanska-Konkel M; Department of Therapy Monitoring and Pharmacogenetics, Medical University of Gdansk, Gdansk, Poland;
  • Jankowski M; Department of Clinical Chemistry, Medical University of Gdansk, Gdansk, Poland; and Laboratory of Molecular and Cellular Nephrology, Mossakowski Medical Research Centre Polish Academy of Sciences, Gdansk, Poland majank@gumed.edu.pl.
Am J Physiol Renal Physiol ; 311(1): F103-11, 2016 07 01.
Article en En | MEDLINE | ID: mdl-27076649
ABSTRACT
Purinoceptors (adrengeric receptors and P2 receptors) are expressed on the cellular components of the glomerular filtration barrier, and their activation may affect glomerular permeability to albumin, which may ultimately lead to albuminuria, a well-established risk factor for the progression of chronic kidney disease and development of cardiovascular diseases. We investigated the mechanisms underlying the in vitro and in vivo purinergic actions on glomerular filter permeability to albumin by measuring convectional albumin permeability (Palb) in a single isolated rat glomerulus based on the video microscopy method. Primary cultured rat podocytes were used for the analysis of Palb, cGMP accumulation, PKG-Iα dimerization, and immunofluorescence. In vitro, natural nucleotides (ATP, ADP, UTP, and UDP) and nonmetabolized ATP analogs (2-meSATP and ATP-γ-S) increased Palb in a time- and concentration-dependent manner. The effects were dependent on P2 receptor activation, nitric oxide synthase, and cytoplasmic guanylate cyclase. ATP analogs significantly increased Palb, cGMP accumulation, and subcortical actin reorganization in a PKG-dependent but nondimer-mediated route in cultured podocytes. In vivo, 2-meSATP and ATP-γ-S increased Palb but did not significantly affect urinary albumin excretion. Both agonists enhanced the clathrin-mediated endocytosis of albumin in podocytes. A product of adenine nucleotides hydrolysis, adenosine, increased the permeability of the glomerular barrier via adrenergic receptors in a dependent and independent manner. Our results suggest that the extracellular nucleotides that stimulate an increase of glomerular Palb involve nitric oxide synthase and cytoplasmic guanylate cyclase with actin reorganization in podocytes.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Purinas / Albúminas / Albuminuria / Glomérulos Renales Tipo de estudio: Etiology_studies / Risk_factors_studies Idioma: En Revista: Am J Physiol Renal Physiol Asunto de la revista: FISIOLOGIA / NEFROLOGIA Año: 2016 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Purinas / Albúminas / Albuminuria / Glomérulos Renales Tipo de estudio: Etiology_studies / Risk_factors_studies Idioma: En Revista: Am J Physiol Renal Physiol Asunto de la revista: FISIOLOGIA / NEFROLOGIA Año: 2016 Tipo del documento: Article