A screening algorithm for diagnosing bacterial gastroenteritis by real-time PCR in combination with guided culture.
Diagn Microbiol Infect Dis
; 85(2): 255-9, 2016 Jun.
Article
en En
| MEDLINE
| ID: mdl-27107537
ABSTRACT
We have introduced a real-time PCR for the simultaneous detection of Campylobacter jejuni, Salmonella spp., Shigella spp./enteroinvasive Escherichia coli and Yersinia enterocolitica in fecal samples in our routine laboratory. This new approach showed consistent results, with minimal inter-sample variation. When compared to conventional culture, the hands-on time decreased by 13 h/wk, and the median turnaround time drastically shortened from 73 to 29 h (P < .0001). Moreover, the detection rate of the targeted pathogens seemed to increase the positivity rate registered over a twelve month period increased from 4.98% when using bacterial culture, compared to 8.56% when using real-time PCR (P < .0001). For antimicrobial susceptibility testing, samples that are found to be PCR positive are additionally cultured after the PCR result is known. Using this algorithm, we got a positive culture for 71.0% of the PCR positive samples. The samples missed by guided culture had significantly higher quantification cycle (Cq) values compared to the samples picked up by guided culture (P = .0003). Finally; we also tested the effect of extended sample storage on the performance of guided culture. Storage time prior to inoculation did have an effect on the positivity rate of culture; interestingly, these effects were clearly species-dependent.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Bacterias
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Infecciones Bacterianas
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Tamizaje Masivo
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Técnicas Bacteriológicas
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Heces
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Reacción en Cadena en Tiempo Real de la Polimerasa
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Gastroenteritis
Tipo de estudio:
Diagnostic_studies
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Evaluation_studies
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Observational_studies
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Prognostic_studies
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Screening_studies
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Año:
2016
Tipo del documento:
Article