Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.
Int J Biol Macromol
; 89: 219-27, 2016 Aug.
Article
en En
| MEDLINE
| ID: mdl-27131730
ABSTRACT
In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography. The D. officinale polysaccharide (DOP) has a molecular weight of 8500Da. Monosaccharide composition analysis reveals that DOP is composed of mannose, glucose, and arabinose with a trace of galacturonic acid in a molar ratio of 6.22.32.10.1. Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1â4)-linked-ß-d-mannopyranosyl and (1â4)-linked-ß-d-glucopyranosyl residues. Atomic force microscope shows that DOP mainly exists as rod-shaped chains, supporting high degrees of polymerization. The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
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Base de datos:
MEDLINE
Asunto principal:
Picratos
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Polisacáridos
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Compuestos de Bifenilo
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Dendrobium
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Antioxidantes
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2016
Tipo del documento:
Article