Osthole inhibits histamine-dependent itch via modulating TRPV1 activity.
Sci Rep
; 6: 25657, 2016 05 10.
Article
en En
| MEDLINE
| ID: mdl-27160770
ABSTRACT
Osthole, an active coumarin isolated from Cnidium monnieri (L.) Cusson, has long been used in China as an antipruritic herbal medicine; however, the antipruitic mechanism of osthole is unknown. We studied the molecular mechanism of osthole in histamine-dependent itch by behavioral test, Ca(2+) imaging, and electrophysiological experiments. First, osthole clearly remitted the scratching behaviors of mice induced with histamine, HTMT, and VUF8430. Second, in cultured dorsal root ganglion (DRG) neurons, osthole showed a dose-dependent inhibitory effect to histamine. On the same neurons, osthole also decreased the response to capsaicin and histamine. In further tests, the capsaicin-induced inward currents were inhibited by osthole. These results revealed that osthole inhibited histamine-dependent itch by modulating TRPV1 activity. This study will be helpful in understanding how osthole exerts anti-pruritus effects and suggests that osthole may be a useful treatment medicine for histamine-dependent itch.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Prurito
/
Activación del Canal Iónico
/
Cumarinas
/
Canales Catiónicos TRPV
Idioma:
En
Revista:
Sci Rep
Año:
2016
Tipo del documento:
Article