Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor.
Nat Commun
; 7: 11645, 2016 05 20.
Article
en En
| MEDLINE
| ID: mdl-27198602
ABSTRACT
Alternative splicing generates protein diversity essential for neuronal properties. However, the precise mechanisms underlying this process and its relevance to physiological and behavioural functions are poorly understood. To address these issues, we focused on a cassette exon of the Caenorhabditis elegans insulin receptor gene daf-2, whose proper variant expression in the taste receptor neuron ASER is critical for taste-avoidance learning. We show that inclusion of daf-2 exon 11.5 is restricted to specific neuron types, including ASER, and is controlled by a combinatorial action of evolutionarily conserved alternative splicing factors, RBFOX, CELF and PTB families of proteins. Mutations of these factors cause a learning defect, and this defect is relieved by DAF-2c (exon 11.5+) isoform expression only in a single neuron ASER. Our results provide evidence that alternative splicing regulation of a single critical gene in a single critical neuron is essential for learning ability in an organism.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Reacción de Prevención
/
Receptor de Insulina
/
Células Quimiorreceptoras
/
Empalme Alternativo
/
Proteínas de Caenorhabditis elegans
/
Factor de Empalme Asociado a PTB
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2016
Tipo del documento:
Article