Blocking the phosphatidylinositol 3-kinase pathway inhibits neuregulin-1-mediated rescue of neurotoxicity induced by Aß1-42.
J Pharm Pharmacol
; 68(8): 1021-9, 2016 Aug.
Article
en En
| MEDLINE
| ID: mdl-27230708
ABSTRACT
OBJECTIVES:
Neuregulin-1 (NRG1) has an important role in both the development and the plasticity of the brain as well as neuroprotective properties. In this study, we investigated the downstream pathways of NRG1 signalling and their role in the prevention of Aß1-42 -induced neurotoxicity.METHODS:
Lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) generation, superoxide dismutase (SOD) activity and TUNEL staining were assayed to examine the neuroprotective properties in primary rat cortical neurons. KEYFINDINGS:
The inhibition of PI3K/Akt activation abolished the ability of NRG1 to prevent Aß1-42 -induced LDH release and increased TUNEL-positive cell count and reactive oxygen species accumulation in primary cortical neurons.CONCLUSIONS:
Our results demonstrate that NRG1 signalling exerts a neuroprotective effect against Aß1-42 -induced neurotoxicity via activation of the PI3K/Akt pathway. Furthermore, this suggests that NRG1 has neuroprotective potential for the treatment of AD.Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Fragmentos de Péptidos
/
Encéfalo
/
Péptidos beta-Amiloides
/
Fármacos Neuroprotectores
/
Neurregulina-1
/
Fosfatidilinositol 3-Quinasa
/
Enfermedad de Alzheimer
Idioma:
En
Revista:
J Pharm Pharmacol
Año:
2016
Tipo del documento:
Article