Biosequestration of copper by bacteria isolated from an abandoned mine by using microbially induced calcite precipitation.
J Gen Appl Microbiol
; 62(4): 206-12, 2016 Sep 12.
Article
en En
| MEDLINE
| ID: mdl-27488956
ABSTRACT
Abandoned mine sites are frequently polluted with high concentrations of heavy metals. In this study, 25 calcite-forming bacteria were newly isolated from the soil of an abandoned metal mine in Korea. Based on their urease activity, calcite production, and resistance to copper toxicity, four isolates were selected and further identified by 16S rRNA gene sequencing. Among the isolates, Sporosarcina soli B-22 was selected for subsequent copper biosequestration studies, using the sand impermeability test by production of calcite and extracellular polymeric substance. High removal rates (61.8%) of copper were obtained when the sand samples were analyzed using an inductively coupled plasma-optical emission spectrometer following 72 h of incubation. Scanning electron microscopy showed that the copper carbonate precipitates had a diameter of approximately 5-10 µm. X-ray diffraction further confirmed the presence of copper carbonate and calcium carbonate crystals.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Microbiología del Suelo
/
Bacterias
/
Carbonato de Calcio
/
Carbonatos
/
Cobre
/
Sporosarcina
País/Región como asunto:
Asia
Idioma:
En
Revista:
J Gen Appl Microbiol
Año:
2016
Tipo del documento:
Article