Recombinant Reg3ß protein protects against streptozotocin-induced ß-cell damage and diabetes.
Sci Rep
; 6: 35640, 2016 10 21.
Article
en En
| MEDLINE
| ID: mdl-27767186
ABSTRACT
Regenerating genes (Reg) have been found during the search for factors involved in pancreatic islet regeneration. Our recent study discovered that pancreatic ß-cell-specific overexpression of Reg3ß protects against streptozotocin (Stz) -induced diabetes in mice. To investigate its potential roles in the treatment of diabetes, we produced a recombinant Reg3ß protein and provided evidence that it is active in promoting islet ß-cell survival against Stz- triggered cell death. Though ineffective in alleviating preexisting diabetes, pretreatment of recombinant Reg3ß was capable of minimizing the Stz-induced hyperglycemia and weight loss, by preserving serum and pancreatic insulin levels, and islet ß-cell mass. No obvious changes were observed in the rate of cell proliferation and hypertrophy in α- or acinar-cells after treatment with recombinant Reg3ß. The underlying mechanism of Reg3ß-mediated protection seems to involve Akt activation which upregulates Bcl-2 and Bcl-xL levels and consequently promotes cell survival.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Diabetes Mellitus Experimental
/
Células Secretoras de Insulina
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Proteínas Asociadas a Pancreatitis
Idioma:
En
Revista:
Sci Rep
Año:
2016
Tipo del documento:
Article