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Propidium monoazide and Xpert MTB/RIF to quantify Mycobacterium tuberculosis cells.
Kayigire, Xavier A; Friedrich, Sven O; Karinja, Miriam N; van der Merwe, Lize; Martinson, Neil A; Diacon, Andreas H.
Afiliación
  • Kayigire XA; Division of Molecular Biology and Human Genetics, MRC Centre for Tuberculosis Research, DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa; Task Applied Science, Bellville, Cape Town, South Afri
  • Friedrich SO; Division of Medical Physiology, MRC Centre for Tuberculosis Research, DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa; Task Applied Science, Bellville, Cape Town, South Africa.
  • Karinja MN; Task Applied Science, Bellville, Cape Town, South Africa.
  • van der Merwe L; Task Applied Science, Bellville, Cape Town, South Africa.
  • Martinson NA; Perinatal HIV Research Unit, MRC Soweto Matlosana Collaborating Centre for HIV/AIDS and TB Research, DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, University of the Witwatersrand, Johannesburg, South Africa.
  • Diacon AH; Division of Medical Physiology, MRC Centre for Tuberculosis Research, DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa; Task Applied Science, Bellville, Cape Town, South Africa. Electronic add
Tuberculosis (Edinb) ; 101: 79-84, 2016 12.
Article en En | MEDLINE | ID: mdl-27865403
Propidium monoazide (PMA) penetrates non-viable cells with compromised membranes. PMA has been proposed to improve the specificity of Xpert MTB/RIF (Xpert) for the detection of viable Mycobacterium tuberculosis. This study assessed the effect of PMA on Xpert cycle thresholds (CT) of M. tuberculosis made non-viable under antibiotic pressure. In vitro, we measured the difference between CT with and without PMA (ΔCT) in liquid cultures treated with one of six anti-tuberculosis drugs (isoniazid, rifampin, pyrazinamide, ethambutol, streptomycin, moxifloxacin) and found significant ΔCT only with isoniazid and ethambutol for pan-susceptible M. tuberculosis and only with ethambutol for extensively drug-resistant M. tuberculosis. In the clinic we assessed ΔCT in sputum samples collected from patients with pulmonary tuberculosis before and at regular intervals over 12 weeks after initiation of treatment. Before treatment start, estimated CT were 19.3 (95% CI: 17.1-21.4) and 19.8 (95% CI: 17.6-22.1) without and with PMA, respectively. Under treatment CT increased by 2.54 per √√day (95% CI: 1.38-3.69) without PMA and an additional 0.55 per √√day (95% CI: 0.37-0.74; p < 0.0001) with PMA. We conclude that PMA increases the specificity of Xpert for viable M. tuberculosis but the effect is small and dependent on the antibiotics used.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Propidio / Azidas / Tuberculosis Pulmonar / Mycobacterium tuberculosis Tipo de estudio: Diagnostic_studies / Observational_studies Idioma: En Revista: Tuberculosis (Edinb) Año: 2016 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Propidio / Azidas / Tuberculosis Pulmonar / Mycobacterium tuberculosis Tipo de estudio: Diagnostic_studies / Observational_studies Idioma: En Revista: Tuberculosis (Edinb) Año: 2016 Tipo del documento: Article