Differential expression of the epithelial mesenchymal transition factors Snail, Slug, Twist, TGF-ß, and E-cadherin in ameloblastoma.
Med Mol Morphol
; 50(2): 68-75, 2017 Jun.
Article
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| MEDLINE
| ID: mdl-27995335
ABSTRACT
Epithelial mesenchymal transition (EMT), the transition of epithelial cells into motile mesenchymal cells, plays an important role in embryogenesis, cancer invasion, and metastasis. Ameloblastomas are common epithelial odontogenic tumors, occurring exclusively in the mandible with locally invasive growth. Thirty-seven ameloblastoma cases were evaluated for the involvement of EMT by immunohistochemical staining and western blotting using antibodies against Slug, Snail, Twist, TGF-ß, and E-cadherin. Double immunostaining was also performed. Slug and TGF-ß were expressed in the nuclei of peripheral and stellate reticulum cells of ameloblastoma nests. Twenty cases of Snail, 36 of Slug, 8 of Twist, and 19 of TGF-ß showed strong expression in tumor cells in follicular and plexiform patterns. Expression of Slug and TGF-ß increased in regions where the expression of E-cadherin was reduced. EMT was found to be associated with the local invasive growth of ameloblastoma. These data suggest that reduced expression of E-cadherin and over-expression of Slug, Snail, and TGF-ß induce EMT. Given that ameloblastomas are characterized by local invasiveness, EMT might be related to their development. Thus, strong expression of Slug and TGF-ß and reduced expression of E-cadherin might be related to the local invasiveness of ameloblastoma.
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Base de datos:
MEDLINE
Asunto principal:
Ameloblastoma
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Neoplasias Mandibulares
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Cadherinas
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Regulación Neoplásica de la Expresión Génica
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Factor de Crecimiento Transformador beta1
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Transición Epitelial-Mesenquimal
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Factores de Transcripción de la Familia Snail
Idioma:
En
Revista:
Med Mol Morphol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
PATOLOGIA
Año:
2017
Tipo del documento:
Article