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Induced Genome-Wide Binding of Three Arabidopsis WRKY Transcription Factors during Early MAMP-Triggered Immunity.
Birkenbihl, Rainer P; Kracher, Barbara; Roccaro, Mario; Somssich, Imre E.
Afiliación
  • Birkenbihl RP; Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Koeln, Germany.
  • Kracher B; Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Koeln, Germany.
  • Somssich IE; Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Koeln, Germany somssich@mpipz.mpg.de.
Plant Cell ; 29(1): 20-38, 2017 01.
Article en En | MEDLINE | ID: mdl-28011690
During microbial-associated molecular pattern-triggered immunity (MTI), molecules derived from microbes are perceived by cell surface receptors and upon signaling to the nucleus initiate a massive transcriptional reprogramming critical to mount an appropriate host defense response. WRKY transcription factors play an important role in regulating these transcriptional processes. Here, we determined on a genome-wide scale the flg22-induced in vivo DNA binding dynamics of three of the most prominent WRKY factors, WRKY18, WRKY40, and WRKY33. The three WRKY factors each bound to more than 1000 gene loci predominantly at W-box elements, the known WRKY binding motif. Binding occurred mainly in the 500-bp promoter regions of these genes. Many of the targeted genes are involved in signal perception and transduction not only during MTI but also upon damage-associated molecular pattern-triggered immunity, providing a mechanistic link between these functionally interconnected basal defense pathways. Among the additional targets were genes involved in the production of indolic secondary metabolites and in modulating distinct plant hormone pathways. Importantly, among the targeted genes were numerous transcription factors, encoding predominantly ethylene response factors, active during early MTI, and WRKY factors, supporting the previously hypothesized existence of a WRKY subregulatory network. Transcriptional analysis revealed that WRKY18 and WRKY40 function redundantly as negative regulators of flg22-induced genes often to prevent exaggerated defense responses.
Asunto(s)

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Arabidopsis / Genoma de Planta / Proteínas de Arabidopsis Idioma: En Revista: Plant Cell Asunto de la revista: BOTANICA Año: 2017 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Arabidopsis / Genoma de Planta / Proteínas de Arabidopsis Idioma: En Revista: Plant Cell Asunto de la revista: BOTANICA Año: 2017 Tipo del documento: Article