Exosomal miR-486 regulates hypoxia-induced erythroid differentiation of erythroleukemia cells through targeting Sirt1.
Exp Cell Res
; 351(1): 74-81, 2017 02 01.
Article
en En
| MEDLINE
| ID: mdl-28043832
ABSTRACT
MicroRNAs (miRNAs) regulate the hypoxia-induced erythroid differentiation of hematopoietic cells. In this study, we identified that miR-486 was a rapid response miRNA to hypoxia in erythroleukemia TF-1 cells. Hypoxia exposure increased both intracellular and miR-486 levels of TF-1 cells. Ectopic miR-486 expression enhanced the growth and erythroid differentiation of TF-1 cells, whereas miR-486 inhibition suppressed their growth and erythroid differentiation. Treatment of TF-1 and cord blood CD34+ cells with exogenous containing miR-486 resulted in an increase of intracellular miR-486 level and enhanced erythroid differentiation. Furthermore, we identified that Sirt1 is a miR-486 target gene which modulates hypoxia-induced erythroid differentiation of TF-1 cells. Thus we identified a novel miRNA regulatory network that contributes to hypoxia-induced erythroid differentiation of hematopoietic cells.
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MEDLINE
Asunto principal:
Leucemia Eritroblástica Aguda
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Células Precursoras Eritroides
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MicroARNs
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Eritropoyesis
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Sirtuina 1
Idioma:
En
Revista:
Exp Cell Res
Año:
2017
Tipo del documento:
Article