Inhibition of microRNA-153 protects neurons against ischemia/reperfusion injury in an oxygen-glucose deprivation and reoxygenation cellular model by regulating Nrf2/HO-1 signaling.
J Biochem Mol Toxicol
; 31(7)2017 Jul.
Article
en En
| MEDLINE
| ID: mdl-28245071
ABSTRACT
MicroRNAs are emerging as critical regulators in cerebral ischemia/reperfusion injury; however, their exact roles remain poorly understood. miR-153 is reported to be a neuron-related miRNA involved in neuroprotection. In this study, we aimed to investigate the precise role of miR-153 in regulating neuron survival during cerebral ischemia/reperfusion injury using an oxygen-glucose deprivation and reoxygenation (OGD/R) cellular model. We found that miR-153 was significantly upregulated in neurons subjected to OGD/R treatment. Inhibition of miR-153 significantly attenuated OGD/R-induced injury and oxidative stress in neurons. Nuclear factor erythroid 2-related factor 2 (Nrf2) was identified as a target gene of miR-153. Inhibition of miR-153 significantly promoted the expression of Nrf2 and heme oxygenase-1 (HO-1). However, silencing of Nrf2 significantly blocked the protective effects of miR-153 inhibition. Our study indicates that the inhibition of miR-153 protects neurons against OGD/R-induced injury by regulating Nrf2/HO-1 signaling and suggests a potential therapeutic target for cerebral ischemia/reperfusion injury.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Daño por Reperfusión
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Transducción de Señal
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MicroARNs
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Hemo-Oxigenasa 1
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Factor 2 Relacionado con NF-E2
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Modelos Neurológicos
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Neuronas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Biochem Mol Toxicol
Asunto de la revista:
BIOLOGIA MOLECULAR
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BIOQUIMICA
/
TOXICOLOGIA
Año:
2017
Tipo del documento:
Article