The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System.
J Vis Exp
; (120)2017 02 28.
Article
en En
| MEDLINE
| ID: mdl-28287531
ABSTRACT
Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Here, we produced iPS cells from mouse amniotic fluid cells, using a non-viral-based transposon system. All obtained iPS cell lines exhibited characteristics of pluripotent cells, including the ability to differentiate toward derivatives of all three germ layers in vitro and in vivo. This strategy opens up the possibility of using cells from diseased fetuses to develop new therapies for birth defects.
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Base de datos:
MEDLINE
Asunto principal:
Preñez
/
Células Madre Pluripotentes Inducidas
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Líquido Amniótico
Idioma:
En
Revista:
J Vis Exp
Año:
2017
Tipo del documento:
Article