Quantification of Methylenecyclopropyl Compounds and Acyl Conjugates by UPLC-MS/MS in the Study of the Biochemical Effects of the Ingestion of Canned Ackee (Blighia sapida) and Lychee (Litchi chinensis).
J Agric Food Chem
; 65(12): 2603-2608, 2017 Mar 29.
Article
en En
| MEDLINE
| ID: mdl-28290200
ABSTRACT
Consumption of ackee (Blighia sapida) and lychee (Litchi chinensis) fruit has led to severe poisoning. Considering their expanded agricultural production, toxicological evaluation has become important. Therefore, the biochemical effects of eating 1 g/kg canned ackee, containing 99.2 µmol/kg hypoglycin A, and 5 g/kg canned lychee, containing 1.3 µmol/kg hypoglycin A, were quantified in a self-experiment. Using ultra-high-performance liquid chromatography/mass spectrometry, hypoglycin A, methylenecyclopropylacetyl-glycine, and methylenecyclopropylformyl-glycine, as well as the respective carnitine conjugates, were found in urine after ingesting ackee. Hypoglycin A and its glycine derivative were also present in urine after eating lychee. Excretion of physiological acyl conjugates was significantly increased in the ackee experiment. Ingestion of ackee led to up to 15.1 nmol/L methylenecyclopropylacetyl-glycine and traces of methylenecyclopropylformyl-carnitine in the serum. These compounds were not found in the serum after eating lychee. Hypoglycin A accumulated in the serum in both experiments.
Palabras clave
Acyl-CoA dehydrogenase deficiency; Jamaican vomiting disease; Litchi; Sapindaceae; ackee poisoning; hypoglycin A; inhibition of ß-oxidation; lychee; methylenecyclopropylacetyl-carnitine; methylenecyclopropylacetyl-glycine; methylenecyclopropylformyl-carnitine; methylenecyclopropylformyl-glycine; methylenecyclopropylglycine
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Contaminación de Alimentos
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Blighia
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Litchi
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Alimentos en Conserva
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Frutas
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Hipoglicinas
Idioma:
En
Revista:
J Agric Food Chem
Año:
2017
Tipo del documento:
Article