Simultaneous LC-MS-MS Determination of Lopinavir and Rifabutin in Human Plasma.
J Chromatogr Sci
; 55(6): 617-624, 2017 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-28334925
ABSTRACT
Tuberculosis (TB) with human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome represents the most common infectious diseases worldwide. Anti-TB drugs are used concurrently with antiretroviral drug for treatment of TB-HIV co-morbidities. Due to lower risk of interaction with protease inhibitors, rifabutin is preferred over rifampicin in treatment of HIV and TB co-morbidity. A simple and specific liquid chromatography tandem mass spectrometry method was developed for quantification of rifabutin (RBT) and lopinavir (LPV) simultaneously in human plasma. Following extraction using 60% n-hexane in ethyl acetate, the processed samples were chromatographed on a Discovery HS C18 column (5 µm, 50 × 4.6 mm, id) using mobile phase [85% acetonitrile in ammonium acetate buffer (10 mM, pH 4.5)] at a flow rate of 0.7 mL/min. Mass spectrometric detection was performed in positive electrospray ionization mode using multiple reaction monitoring (RBT, m/z 847.7 â 815.4; LPV, m/z 629.6 â 447.4). Raloxifene and phenacetin were used as internal standards for RBT and LPV, respectively. Linearity was established in the range of 1-1,000 ng/mL and 0.5-10 µg/mL (R2 ≥ 0.99) for RBT and LPV, respectively. The recovery of LPV and RBT were always >90 and >50%, respectively. The precisions and accuracies were well within the acceptable limits of variation.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Cromatografía Liquida
/
Rifabutina
/
Espectrometría de Masas en Tándem
/
Lopinavir
Tipo de estudio:
Diagnostic_studies
/
Guideline
/
Prognostic_studies
Idioma:
En
Revista:
J Chromatogr Sci
Año:
2017
Tipo del documento:
Article