Natural product inhibitors of carbonic anhydrase I and II isoenzymes: osajin and pomiferin.
Arch Physiol Biochem
; 123(4): 219-224, 2017 Oct.
Article
en En
| MEDLINE
| ID: mdl-28338341
ABSTRACT
The aim of this study is to purify carbonic anhydrase I and II isoenzymes from human erythrocyte, isolate two natural products osajin (OSJ) and pomiferin (PMF) from Maclura pomifera fruits, and evaluate the in vitro effect of these natural metabolites on these isoenzymes. These natural products may be used as starting points for drug discovery (like drugs used in several therapeutic applications, including antiglaucoma activity). For the purification procedure, the Sepharose-4B-l-tyrosine-sulphonamide affinity chromatography was used. Column chromatography and thin layer chromatography methods were used for isolation of OSJ and PMF from M. pomifera fruits and their chemical structures were elucidated by IR, 1D, and 2D NMR methods. We compared inhibitory effects of these natural products with inhibitory effects of phenolic compounds and found that these products demonstrated average inhibition effects. We thought that this study will give inspiration to scientists interested in this issue.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Benzopiranos
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Productos Biológicos
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Inhibidores de Anhidrasa Carbónica
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Extractos Vegetales
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Anhidrasa Carbónica I
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Anhidrasa Carbónica II
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Eritrocitos
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Isoflavonas
Idioma:
En
Revista:
Arch Physiol Biochem
Asunto de la revista:
BIOQUIMICA
/
FISIOLOGIA
Año:
2017
Tipo del documento:
Article