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A Cleanup Method for Mass Spectrometric Analysis of Sphingosine- and Ceramide-1-Phosphate in Blood and Solid Tissue Using a Phosphate Capture Molecule.
Morishige, Jun-Ichi; Yamashita, Ryouhei; Tanaka, Tamotsu; Satouchi, Kiyoshi.
Afiliación
  • Morishige JI; Department of Cellular and Molecular Function Analysis, Kanazawa University Graduate School of Medical Science, Kanazawa, 920-8640, Japan.
  • Yamashita R; Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8505, Japan.
  • Tanaka T; Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8505, Japan. tanaka.tamotsu@tokushima-u.ac.jp.
  • Satouchi K; Department of Applied Biological Science, Fukuyama University, Fukuyama, 729-0292, Japan.
Methods Mol Biol ; 1697: 57-71, 2018.
Article en En | MEDLINE | ID: mdl-28361484
ABSTRACT
Cleanup technology and mass spectrometric determination of sphingosine-1-phosphate (S1P) using a phosphate capture molecule are shown. The protocol is rapid, requires neither thin-layer chromatography nor liquid chromatography, and is applicable to both blood and solid tissue samples. The mass spectrometric method is also applicable to ceramide-1-phosphate.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Esfingosina / Lisofosfolípidos / Ceramidas / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article
Texto completo
Imprimir
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Esfingosina / Lisofosfolípidos / Ceramidas / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article