Specific circulating microRNA signature of bicuspid aortic valve disease.
J Transl Med
; 15(1): 76, 2017 04 11.
Article
en En
| MEDLINE
| ID: mdl-28399937
ABSTRACT
BACKGROUND:
We aimed to determine the circulating miRNA expression profile associated with BAV and aortic dilation to provide diagnostic and prognostic biomarkers for BAV and/or aortic dilation. METHODS ANDRESULTS:
We applied a miRNome-wide microarray approach using plasma samples (n = 24) from healthy tricuspid aortic valve individuals, BAV patients and BAV patients with aortic dilation to compare and identify the specific miRNAs associated with BAV and aortic dilation. In a second stage, the expression patterns of the miRNA candidates were validated by RT-qPCR in an independent cohort (n = 43). The miRNA microarray data and RT-qPCR analyses revealed that the expression levels of circulating miR-122, miR-130a and miR-486 are significantly influenced by the morphology of the aortic valve (bicuspid/tricuspid) and could be functionally involved in the regulation of TGF-ß1 signalling. Furthermore, the expression pattern of miR-718 in the plasma was strongly influenced by dilation of the ascending aorta. miR-718 expression was inversely correlated with the aortic diameter (R = -0.63, p = 3.1 × 10-5) and was an independent predictor of aortic dilation (ß = -0.41, p = 0.022). The genes targeted by miR-718 are involved in the regulation of vascular remodelling.CONCLUSIONS:
We propose that miR-122, miR-130a, miR-486 and miR-718 are new molecular features associated with BAV and aortic dilation principally by the activation of TGF-ß1 pathway and vascular remodelling mediated by VEGF signalling pathways.Palabras clave
Texto completo:
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Base de datos:
MEDLINE
Asunto principal:
Válvula Aórtica
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Perfilación de la Expresión Génica
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MicroARNs
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Enfermedades de las Válvulas Cardíacas
Tipo de estudio:
Etiology_studies
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Incidence_studies
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Observational_studies
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Prognostic_studies
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Risk_factors_studies
Idioma:
En
Revista:
J Transl Med
Año:
2017
Tipo del documento:
Article