Arabidopsis 14-3-3 epsilon members contribute to polarity of PIN auxin carrier and auxin transport-related development.
Elife
; 62017 04 19.
Article
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| MEDLINE
| ID: mdl-28422008
ABSTRACT
Eukaryotic 14-3-3 proteins have been implicated in the regulation of diverse biological processes by phosphorylation-dependent protein-protein interactions. The Arabidopsis genome encodes two groups of 14-3-3s, one of which - epsilon - is thought to fulfill conserved cellular functions. Here, we assessed the in vivo role of the ancestral 14-3-3 epsilon group members. Their simultaneous and conditional repression by RNA interference and artificial microRNA in seedlings led to altered distribution patterns of the phytohormone auxin and associated auxin transport-related phenotypes, such as agravitropic growth. Moreover, 14-3-3 epsilon members were required for pronounced polar distribution of PIN-FORMED auxin efflux carriers within the plasma membrane. Defects in defined post-Golgi trafficking processes proved causal for this phenotype and might be due to lack of direct 14-3-3 interactions with factors crucial for membrane trafficking. Taken together, our data demonstrate a fundamental role for the ancient 14-3-3 epsilon group members in regulating PIN polarity and plant development.
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Base de datos:
MEDLINE
Asunto principal:
Proteínas de Transporte de Membrana
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Reguladores del Crecimiento de las Plantas
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Arabidopsis
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Proteínas 14-3-3
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Desarrollo de la Planta
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Ácidos Indolacéticos
Idioma:
En
Revista:
Elife
Año:
2017
Tipo del documento:
Article