A mechanistic study of helicases with magnetic traps.
Protein Sci
; 26(7): 1314-1336, 2017 Jul.
Article
en En
| MEDLINE
| ID: mdl-28474797
ABSTRACT
Helicases are a broad family of enzymes that separate nucleic acid double strand structures (DNA/DNA, DNA/RNA, or RNA/RNA) and thus are essential to DNA replication and the maintenance of nucleic acid integrity. We review the picture that has emerged from single molecule studies of the mechanisms of DNA and RNA helicases and their interactions with other proteins. Many features have been uncovered by these studies that were obscured by bulk studies, such as DNA strands switching, mechanical (rather than biochemical) coupling between helicases and polymerases, helicase-induced re-hybridization and stalled fork rescue.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
ADN
/
ARN Bicatenario
/
ADN Helicasas
/
ARN Helicasas
/
Replicación del ADN
/
Ácidos Nucleicos Heterodúplex
Idioma:
En
Revista:
Protein Sci
Asunto de la revista:
BIOQUIMICA
Año:
2017
Tipo del documento:
Article