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Bordetella effector BopN is translocated into host cells via its N-terminal residues.
Abe, Akio; Nishimura, Ryutaro; Kuwae, Asaomi.
Afiliación
  • Abe A; Laboratory of Bacterial Infection, Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
  • Nishimura R; Laboratory of Bacterial Infection, Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
  • Kuwae A; Laboratory of Bacterial Infection, Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Microbiol Immunol ; 61(6): 206-214, 2017 Jun.
Article en En | MEDLINE | ID: mdl-28500733
Bordetella bronchiseptica infects a wide variety of mammals, the type III secretion system (T3SS) being involved in long-term colonization by Bordetella of the trachea and lung. T3SS translocates virulence factors (commonly referred to as effectors) into host cells, leading to alterations in the host's physiological function. The Bordetella effectors BopN and BteA are known to have roles in up-regulation of IL-10 and cytotoxicity, respectively. Nevertheless, the mechanism by which BopN is translocated into host cells has not been examined in sufficient detail. Therefore, to determine the precise mechanisms of translocation of BopN into host cells, truncated derivatives of BopN were built and the derivatives' ability to translocate into host cells evaluated by adenylate cyclase-mediated translocation assay. It was found that N-terminal amino acid (aa) residues 1-200 of BopN are sufficient for its translocation into host cells. Interestingly, BopN translocation was completely blocked by deletion of the N-terminal aa residues 6-50, indicating that the N-terminal region is critical for BopN translocation. Furthermore, BopN appears to play an auxiliary role in BteA-mediated cytotoxicity. Thus, BopN can apparently translocate into host cells and may facilitate activity of BteA.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Infecciones por Bordetella / Bordetella bronchiseptica / Transporte de Proteínas Idioma: En Revista: Microbiol Immunol Año: 2017 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Infecciones por Bordetella / Bordetella bronchiseptica / Transporte de Proteínas Idioma: En Revista: Microbiol Immunol Año: 2017 Tipo del documento: Article