Descemet's Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits.
Sci Rep
; 7(1): 6983, 2017 08 01.
Article
en En
| MEDLINE
| ID: mdl-28765543
ABSTRACT
Descemet's membrane (DM) helps maintain phenotype and function of corneal endothelial cells under physiological conditions, while little is known about the function of DM in corneal endothelial wound healing process. In the current study, we performed in vivo rabbit corneal endothelial cell (CEC) injury via CEC scraping, in which DM remained intact after CECs removal, or via DM stripping, in which DM was removed together with CECs. We found rabbit corneas in the CEC scraping group healed with transparency restoration, while there was posterior fibrosis tissue formation in the corneas after DM stripping on day 14. Following CEC scraping on day 3, cells that had migrated toward the central cornea underwent a transient fibrotic endothelial-mesenchymal transition (EMT) which was reversed back to an endothelial phenotype on day 14. However, in the corneas injured via DM stripping, most of the cells in the posterior fibrosis tissue did not originate from the corneal endothelium, and they maintained fibroblastic phenotype on day 14. We concluded that corneal endothelial wound healing in rabbits has different outcomes depending upon the presence or absence of Descemet's membrane. Descemet's membrane supports corneal endothelial cell regeneration in rabbits after endothelial injury.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Células Endoteliales
/
Lámina Limitante Posterior
/
Lesiones de la Cornea
Idioma:
En
Revista:
Sci Rep
Año:
2017
Tipo del documento:
Article