[MiR-486 Regulates the Glycometabolism of Hematopoietic Cells by Targeting Sirt1].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 25(5): 1283-1288, 2017 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-29070096
ABSTRACT
OBJECTIVE:
To investigate the effect and mechanism of miR-486 on glycometabolism of hematopoietic cells.METHODS:
qRT-PCR was applied to detect the expression of miR-486 or Sirt1 on TF-1 cells under hypoxia. Lentivirus was used to mediate the overexpression or inhibition of miR-486 on TF-1 cells and qRT-PCR was used to detect the expressions of Sirt1, glucose transporter 1(Glut1) and glucose transporter 4(Glut4). Lentivirus-mediated Sirt1-shRNA transduction was used to knockdown Sirt1 expression which was detected by qRT-PCR and Western blot. The expressions of Glut1 and Glut4 were determined by qRT-PCR.RESULTS:
Hypoxia promoted the expression of miR-486 and inhibited the expression of Sirt1. MiR-486 overexpression could inhibit the expression of Sirt1 and promote the expressions of Glut1 and Glut4, whereas miR-486 silencing upregulated the sirt1 expression and inhibited the expressions of Glut1 and Glut4. And inhibition of Sirt1 expression increased the expressions of Glut1 and Glut4.CONCLUSION:
MiR-486 can regulate the glycometabolism of hematopoietic cells by targeting Sirt1.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Células Madre Hematopoyéticas
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MicroARNs
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ARN Interferente Pequeño
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Sirtuina 1
Idioma:
Zh
Revista:
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Asunto de la revista:
HEMATOLOGIA
Año:
2017
Tipo del documento:
Article