Functionally-Relevant Morphological Profiling: A Tool to Assess Cellular Heterogeneity.
Trends Biotechnol
; 36(1): 105-118, 2018 01.
Article
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| MEDLINE
| ID: mdl-29126572
ABSTRACT
Heterogeneity in cell function has presented a significant hurdle to the successful clinical translation of many cellular therapies. Current techniques for assessing cell quality and the effects of microenvironmental cues and manufacturing processes on cell behavior often inadequately address heterogeneity due to issues such as population versus single-cell measurements and the therapeutic relevance and throughput/robustness of the assay. Due to the well-established relationship between morphology and cellular function, morphological profiling has become increasingly utilized to better understand functional heterogeneity and its impact on therapeutic development. In this review, we introduce an emerging field we term functionally-relevant morphological profiling with great potential to improve our understanding of cellular heterogeneity through discovering novel quality attributes, optimizing manufacturing, and screening drugs/biomaterials.
Palabras clave
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1
Base de datos:
MEDLINE
Asunto principal:
Procesamiento de Imagen Asistido por Computador
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Técnicas Citológicas
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Forma de la Célula
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Imagen Óptica
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Microscopía
Idioma:
En
Revista:
Trends Biotechnol
Año:
2018
Tipo del documento:
Article