Control of auxin-induced callus formation by bZIP59-LBD complex in Arabidopsis regeneration.
Nat Plants
; 4(2): 108-115, 2018 02.
Article
en En
| MEDLINE
| ID: mdl-29358751
ABSTRACT
Induction of pluripotent cells termed callus by auxin represents a typical cell fate change required for plant in vitro regeneration; however, the molecular control of auxin-induced callus formation is largely elusive. We previously identified four Arabidopsis auxin-inducible Lateral Organ Boundaries Domain (LBD) transcription factors that govern callus formation. Here, we report that Arabidopsis basic region/leucine zipper motif 59 (AtbZIP59) transcription factor forms complexes with LBDs to direct auxin-induced callus formation. We show that auxin stabilizes AtbZIP59 and enhances its interaction with LBD, and that disruption of AtbZIP59 dampens auxin-induced callus formation whereas overexpression of AtbZIP59 triggers autonomous callus formation. AtbZIP59-LBD16 directly targets a FAD-binding Berberine (FAD-BD) gene and promotes its transcription, which contributes to callus formation. These findings define the AtbZIP59-LBD complex as a critical regulator of auxin-induced cell fate change during callus formation, which provides a new insight into the molecular regulation of plant regeneration and possible developmental programs.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Reguladores del Crecimiento de las Plantas
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Arabidopsis
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Regulación de la Expresión Génica de las Plantas
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Proteínas de Arabidopsis
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Factores de Transcripción con Cremalleras de Leucina de Carácter Básico
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Ácidos Indolacéticos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Nat Plants
Año:
2018
Tipo del documento:
Article