Salusin-ß Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma.
Oxid Med Cell Longev
; 2017: 6905217, 2017.
Article
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| MEDLINE
| ID: mdl-29359008
ABSTRACT
The pathophysiological mechanisms for vascular lesions in diabetes mellitus (DM) are complex, among which endothelial dysfunction plays a vital role. Therapeutic target against endothelial injury may provide critical venues for treatment of diabetic vascular diseases. We recently identified that salusin-ß contributed to high glucose-induced endothelial cell apoptosis. However, the roles of salusin-ß in DM-induced endothelial dysfunction remain largely elusive. Male C57BL/6J mice were used to induce type 2 diabetes mellitus (T2DM) model. Human umbilical vein endothelial cells (HUVECs) were cultured in high glucose/high fat (HG/HF) medium. We demonstrated increased expression of salusin-ß in diabetic aortic tissues and high-glucose/high-fat- (HG/HF-) incubated HUVECs. Disruption of salusin-ß by shRNA abrogated the reactive oxygen species (ROS) production, inflammation, and nitrotyrosine content of HUVECs cultured in HG/HF medium. The HG/HF-mediated decrease in peroxisome proliferator-activated receptor γ (PPARγ) expression was restored by salusin-ß shRNA, and PPARγ inhibitor T0070907 abolished the protective actions of salusin-ß shRNA on endothelial injury in HG/HF-treated HUVECs. Salusin-ß silencing obviously improved endothelium-dependent vasorelaxation, oxidative stress, inflammatory response, and nitrative stress in diabetic aorta. Taken together, our results highlighted the essential role of salusin-ß in pathological endothelial dysfunction, and salusin-ß may be a promising target in treatment of vascular complications of DM.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Enfermedades Vasculares
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Péptidos y Proteínas de Señalización Intercelular
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PPAR gamma
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Diabetes Mellitus Experimental
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Diabetes Mellitus Tipo 2
/
Células Endoteliales de la Vena Umbilical Humana
Tipo de estudio:
Etiology_studies
/
Prognostic_studies
Idioma:
En
Revista:
Oxid Med Cell Longev
Asunto de la revista:
METABOLISMO
Año:
2017
Tipo del documento:
Article