Rearrangement and organization of T cell receptor gamma chain genes in human leukemic T cell lines.
Eur J Immunol
; 17(11): 1653-6, 1987 Nov.
Article
en En
| MEDLINE
| ID: mdl-2960549
ABSTRACT
Seventeen T cell leukemic lines, which are arrested at different stages of differentiation, were analyzed for gamma-chain rearrangement and expression using the constant region gene and representatives of the different families of V gamma chain genes. With the exception of the T cell line P30/OKUBO, which is arrested at the earliest stage (stage I), all the leukemic lines have rearranged their gamma-chain genes and transcripts can be detected in all but MOLT-4 and P30/OKUBO cell lines. Although V gamma 1 genes were rearranged and expressed in leukemic lines arrested at stages II, III and IV, V gamma 2 and V gamma 3 were only rearranged and expressed in cells at stage III (0/5 in stage II, 5/9 in stage III and 0/1 in stage 4). Thus, there may be a sequential usage of V gamma gene during human T cell ontogeny (although it is possible that this may just be a coincidence), similar to that which has been shown during murine ontogeny. Based on the rearrangement data, the genomic order of 5' V gamma 1-V gamma 2-V gamma 3-J-C 3' is proposed.
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Base de datos:
MEDLINE
Asunto principal:
Células Tumorales Cultivadas
/
Receptores de Antígenos de Linfocitos T
/
Linfocitos T
/
Leucemia
Idioma:
En
Revista:
Eur J Immunol
Año:
1987
Tipo del documento:
Article