Advanced maturation of human cardiac tissue grown from pluripotent stem cells.
Nature
; 556(7700): 239-243, 2018 04.
Article
en En
| MEDLINE
| ID: mdl-29618819
Cardiac tissues generated from human induced pluripotent stem cells (iPSCs) can serve as platforms for patient-specific studies of physiology and disease1-6. However, the predictive power of these models is presently limited by the immature state of the cells1, 2, 5, 6. Here we show that this fundamental limitation can be overcome if cardiac tissues are formed from early-stage iPSC-derived cardiomyocytes soon after the initiation of spontaneous contractions and are subjected to physical conditioning with increasing intensity over time. After only four weeks of culture, for all iPSC lines studied, such tissues displayed adult-like gene expression profiles, remarkably organized ultrastructure, physiological sarcomere length (2.2 µm) and density of mitochondria (30%), the presence of transverse tubules, oxidative metabolism, a positive force-frequency relationship and functional calcium handling. Electromechanical properties developed more slowly and did not achieve the stage of maturity seen in adult human myocardium. Tissue maturity was necessary for achieving physiological responses to isoproterenol and recapitulating pathological hypertrophy, supporting the utility of this tissue model for studies of cardiac development and disease.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
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Miocitos Cardíacos
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Técnicas de Cultivo de Tejidos
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Células Madre Pluripotentes Inducidas
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Corazón
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Miocardio
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Nature
Año:
2018
Tipo del documento:
Article