Increased level of cell-derived microparticles in the cyst fluids of odontogenic keratocysts.
Int J Oncol
; 52(6): 1863-1874, 2018 Jun.
Article
en En
| MEDLINE
| ID: mdl-29620170
ABSTRACT
The aim of this study was to examine the level and basic characteristics of cellderived microparticles (MPs) in the cyst fluids of odontogenic keratocysts (OKCs). For this purpose, MPs from the cyst fluids (CFMPs) of OKCs were purified by a classic differential centrifugation method and characterized by a transmission electron microscope and fluorescence microscope. Flow cytometric analysis was used to determine the size, concentration and cellular origins of the CFMPs. Moreover, the expression level of receptor activator for nuclear factorκB ligand in the OKCs was evaluated by immunohistochemical staining and then analyzed for its correlation with the concentration of CFMPs by Spearman's rank correlation test. In addition, reverse transcriptionquantitative polymerase chain reaction (RTqPCR) and tartaricresistant acid phosphatase (TRAP) staining were performed to examine the osteoclastogenesis of mouse bone marrowderived macrophages (BMMs) in response to CFMPs. The results revealed that the levels of total CFMPs were significantly elevated in OKCs compared with dentigerous cysts (DCs) and radicular cysts (RCs). In addition, in vitro experiments further revealed that CFMPs derived from the OKCs of patients could be taken up by BMMs, leading to a significant increase in the mRNA expression levels of nuclear factor of activated Tcells 1 (NFATc1) and TRAP. Moreover, TRAPpositive multinucleated osteoclasts were successfully cultured in the presence of macrophage colonystimulating factor (MCSF) and CFMPs with BMMs. On the whole, our findings indicate that patients with OKCs have higher levels of CFMPs compared with patients with DCs and RCs, which may be associated with the bone resorption of OKCs.
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Base de datos:
MEDLINE
Asunto principal:
Quiste Dentígero
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FN-kappa B
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Factores de Transcripción NFATC
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Micropartículas Derivadas de Células
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Fosfatasa Ácida Tartratorresistente
Idioma:
En
Revista:
Int J Oncol
Asunto de la revista:
NEOPLASIAS
Año:
2018
Tipo del documento:
Article