Validated UHPLC-MS/MS method for quantification of doxycycline in abdominal aortic aneurysm patients.

ABSTRACT

AIM: There is a strong evidence that doxycycline can benefit abdominal aortic aneurysms patients because of its ability to inhibit matrix metalloproteinase enzymes. There is a need for a specific quantification method for doxycycline in these patients. We report herein the development and validation of a selective, specific, simple and rapid UHPLC-MS/MS method for doxycycline. RESULTS: The assay was linear (r2 > 0.994) over a concentration range of 0.010-7.005 µg/ml. This validated method has good lower limit of quantification (LLOQ) (10 ng/ml), accuracies (89.1-103.4%), precision (≤7.0%) and extraction recoveries (95.8%). Doxycycline was stable during short- and long-term storage. CONCLUSION: The assay is reliable and has been successfully applied to serum samples obtained from the patients of N-TA3CT clinical study.