Changes of NK cell subsets with time post-transplant in peripheral blood of renal transplant recipients.
Transpl Immunol
; 49: 59-71, 2018 08.
Article
en En
| MEDLINE
| ID: mdl-29702201
ABSTRACT
BACKGROUND:
There is evidence that NK cells with low cytotoxicity but strong immunoregulatory characteristics contribute to good graft outcome. We attempted to investigate which NK cell subsets increase post-transplant and might affect graft function.METHOD:
Lymphocyte and NK cell subsets were determined in whole blood using eight-colour-fluorescence flow cytometry in patients pre-transplant and post-transplant. In total, 31 transplant recipients were studied.RESULTS:
When cell numbers were compared in 9 patients pre- and 6â¯months post-transplant, post-transplant CD56dimCD16+ (pâ¯=â¯0.011) NK cells with the phenotype CD158a+ (pâ¯=â¯0.008), CD158e+ (pâ¯=â¯0.038), NKG2A+ (pâ¯=â¯0.008), NKG2D+ (pâ¯=â¯0.011), IFNyR+ (pâ¯=â¯0.008), perforin+ (pâ¯=â¯0.008), granzymeB+ (pâ¯=â¯0.008), perforin+granzymeB+ (pâ¯=â¯0.008) and perforin-granzymeB- (pâ¯=â¯0.021) were lower than those pre-transplant, indicating a post-transplant reduction of cytotoxic NK cells. In 28 patients NK cell subsets were analyzed with respect to time post-transplant (median 888â¯days post-transplant). CD56dimCD16+ NK cells co-expressing CD158a (pâ¯=â¯0.014), NKG2D (pâ¯=â¯0.047), IL4R (pâ¯=â¯0.038), IL10R (pâ¯=â¯0.008) and IFNy (pâ¯=â¯0.036) as well as CD56bright NK cells with the phenotype TGFß+ (pâ¯=â¯0.017), TGFR+ (pâ¯=â¯0.035), CD158a+ (pâ¯=â¯0.042) and perforin-granzymeB- (pâ¯=â¯0.048) increased with time post-transplant.CONCLUSION:
Post-transplant, cytotoxic NK cells were lower than pre-transplant and remained low, whereas NK cell subsets with potentially immunoregulatory properties increased.Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Complicaciones Posoperatorias
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Células Asesinas Naturales
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Subgrupos Linfocitarios
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Trasplante de Riñón
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Rechazo de Injerto
Idioma:
En
Revista:
Transpl Immunol
Asunto de la revista:
ALERGIA E IMUNOLOGIA
/
TRANSPLANTE
Año:
2018
Tipo del documento:
Article