Immobilized Ferrous Ion and Glucose Oxidase on Graphdiyne and Its Application on One-Step Glucose Detection.
ACS Appl Mater Interfaces
; 11(3): 2647-2654, 2019 Jan 23.
Article
en En
| MEDLINE
| ID: mdl-29707939
ABSTRACT
Graphdiyne (GDY) is a novel two-dimensional (2D) carbon allotrope with sp-hybridized carbon atoms and hexagonal rings. Because of its unique structure and electronic property, GDY was reported as a promising candidate applied in energy storage, catalysis, biosensing and so on. However, using GDY as a platform to immobilize metal ion or enzyme was still not reported. Here, we presented a GDY-based composite with dual-enzyme activity by immobilizing ferrous ion and glucose oxidase onto GDY sheet. GDY showed great adsorption capacity and maintained the high catalytic activity of ferrous ion. The ferrous ion preferred to adsorb in between the neighboring two C-C triple bonds of GDY with lower adsorption energy (-5.64 eV) if compared to graphene (-1.69 eV). Meanwhile, GDY exhibited the ability of adsorbing glucose oxidase while did not obviously influence the structure and catalytic activity of the enzyme. The as-prepared composite was successfully used in one-step blood glucose detection. This work provides a new insight on ion and enzyme immobilization by 2D material.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Enzimas Inmovilizadas
/
Glucosa
/
Glucosa Oxidasa
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
ACS Appl Mater Interfaces
Asunto de la revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Año:
2019
Tipo del documento:
Article