Shedding of Japanese Encephalitis Virus in Oral Fluid of Infected Swine.
Vector Borne Zoonotic Dis
; 18(9): 469-474, 2018 09.
Article
en En
| MEDLINE
| ID: mdl-29742002
ABSTRACT
Japanese encephalitis virus (JEV) is a zoonotic mosquito-borne flavivirus endemic in the Asia-Pacific region. Maintenance of JEV in nature involves enzootic transmission by competent Culex mosquitoes among susceptible avian and swine species. Historically, JEV has been regarded as one of the most important arthropod-borne viruses in Southeast Asia. Oronasal shedding of JEV from infected amplification hosts was not recognized until the recent discovery of vector-free transmission of JEV among domestic pigs. In this study, oral shedding of JEV was characterized in domestic pigs and miniature swine representing the feral phenotype. A rope-based sampling method followed by the detection of viral RNA using RT-qPCR allowed the collection and detection of JEV in oral fluid samples collected from intradermally challenged animals. The results suggest that the shedding of JEV in oral fluid can be readily detected by molecular diagnostic assays at the acute phase of infection. It also demonstrates the feasibility of this technique for the diagnosis and surveillance of JEV in swine species.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Saliva
/
Enfermedades de los Porcinos
/
Esparcimiento de Virus
/
Encefalitis Japonesa
/
Virus de la Encefalitis Japonesa (Especie)
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Vector Borne Zoonotic Dis
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
/
EPIDEMIOLOGIA
Año:
2018
Tipo del documento:
Article