Genome-wide distribution of linker histone H1.0 is independent of MeCP2.
Nat Neurosci
; 21(6): 794-798, 2018 06.
Article
en En
| MEDLINE
| ID: mdl-29802390
ABSTRACT
Previous studies suggested that MeCP2 competes with linker histone H1, but this hypothesis has never been tested in vivo. Here, we performed chromatin immunoprecipitation followed by sequencing (ChIP-seq) of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 binding was not changed upon MeCP2 depletion. Furthermore, mild overexpression of H1.0 did not alter MeCP2 binding, suggesting that the functional binding of MeCP2 and H1.0 are largely independent.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Histonas
/
Proteína 2 de Unión a Metil-CpG
Idioma:
En
Revista:
Nat Neurosci
Asunto de la revista:
NEUROLOGIA
Año:
2018
Tipo del documento:
Article