Overexpression of Fra-1, c-Jun and c-Fos in odontogenic keratocysts: potential correlation with proliferative and anti-apoptotic activity.
Histopathology
; 73(6): 933-942, 2018 Dec.
Article
en En
| MEDLINE
| ID: mdl-29993138
ABSTRACT
AIMS:
The purpose of this study was to explore the potential involvement of Fra-1, c-Jun and c-Fos, three vital members of the AP-1 complex, in the pathogenesis of odontogenic keratocysts (OKCs). METHODS ANDRESULTS:
Tissue samples, containing 10 normal oral mucosa (OM), 10 dentigerous cysts (DC) and 32 OKC specimens, were applied to investigate the expression levels of Fra-1, c-Jun and c-Fos by immunohistochemistry and real-time-quantitative polymerase chain reaction (RT-qPCR). The association between Fra-1, c-Jun and c-Fos expression levels and markers of proliferation [Ki-67, proliferating cell nuclear antigen (PCNA)], anti-apoptosis (Bcl-2) was then investigated in the OKC serial tissue sections. The results showed that Fra-1, c-Jun and c-Fos expression levels were increased significantly in OKCs compared to these in OM and DC tissue samples. Meanwhile, the expression levels of Fra-1, c-Jun and c-Fos were associated positively with the expression levels of Ki-67, PCNA and Bcl-2, as confirmed further by double-labelling immunofluorescence analysis and hierarchical analysis.CONCLUSIONS:
This study revealed for the first time that Fra-1, c-Jun and c-Fos were overexpressed in OKCs and had a close correlation with proliferation and anti-apoptosis potential of OKCs.Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Quistes Odontogénicos
/
Proteínas Proto-Oncogénicas c-jun
/
Proteínas Proto-Oncogénicas c-fos
/
Apoptosis
/
Proliferación Celular
/
Mucosa Bucal
Idioma:
En
Revista:
Histopathology
Año:
2018
Tipo del documento:
Article