Characterizing the steroidal milieu in amniotic fluid of mid-gestation: A LC-MS/MS study.
J Steroid Biochem Mol Biol
; 185: 47-56, 2019 01.
Article
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| MEDLINE
| ID: mdl-30031148
ABSTRACT
Growth and development of an embryo or fetus during human pregnancy mainly depend on intact hormone biosynthesis and metabolism in maternal amniotic fluid (AF). We investigated the hormonal milieu in AF and developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of 14 sulfated and 6 unconjugated steroids in AF. 65â¯Aâ¯F samples (male femaleâ¯=â¯35 30) of mid-gestation ranging from 16th week of gestation to 25th week of gestation were analyzed. Reference data of 20 steroid levels in AF of healthy women were provided. 13 sulfated and 3 unconjugated steroids were for the first time quantified in AF by LC-MS/MS. Highest concentrations were found for pregnenolone sulfate (PregS mean⯱â¯SD, 8.6⯱â¯3.7â¯ng/mL), 17α-hydroxypregnenolone sulfate (17OHPregS 4.9⯱â¯2.0â¯ng/mL), epitestosterone sulfate (eTS 7.3⯱â¯3.6â¯ng/mL), 16α-hydroxydehydroepiandrosterone sulfate (16OH-DHEAS 21.5⯱â¯10.7â¯ng/mL), androsterone sulfate (AnS 9.2⯱â¯7.4â¯ng/mL), estrone sulfate (E1S 3.0⯱â¯3.0â¯ng/mL), estriol 3-sulfate (E3S 8.1⯱â¯4.0â¯ng/mL) and estriol (E3 1.2⯱â¯0.4â¯ng/mL). Only testosterone (T) showed a significant sex difference (pâ¯<â¯0.0001). Correlations between AF steroids mirrored the steroid metabolism of the feto-placental unit, and not only confirmed the classical steroid pathway, but also pointed to a sulfated steroid pathway.
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Texto completo:
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Base de datos:
MEDLINE
Asunto principal:
Segundo Trimestre del Embarazo
/
Esteroides
/
Líquido Amniótico
Idioma:
En
Revista:
J Steroid Biochem Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
2019
Tipo del documento:
Article