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Principles and characteristics of the Arabidopsis WRKY regulatory network during early MAMP-triggered immunity.
Birkenbihl, Rainer P; Kracher, Barbara; Ross, Annegret; Kramer, Katharina; Finkemeier, Iris; Somssich, Imre E.
Afiliación
  • Birkenbihl RP; Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linné Weg 10, 50829, Cologne, Germany.
  • Kracher B; Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linné Weg 10, 50829, Cologne, Germany.
  • Ross A; Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linné Weg 10, 50829, Cologne, Germany.
  • Kramer K; Plant Proteomics, Max Planck Institute for Plant Breeding Research, Carl-von-Linné Weg10, 50829, Cologne, Germany.
  • Finkemeier I; Plant Proteomics, Max Planck Institute for Plant Breeding Research, Carl-von-Linné Weg10, 50829, Cologne, Germany.
  • Somssich IE; Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linné Weg 10, 50829, Cologne, Germany.
Plant J ; 96(3): 487-502, 2018 11.
Article en En | MEDLINE | ID: mdl-30044528
During microbe-associated molecular pattern-triggered immunity more than 5000 Arabidopsis genes are significantly altered in their expression, and the question arises, how such an enormous reprogramming of the transcriptome can be regulated in a safe and robust manner? For the WRKY transcription factors (TFs), which are important regulators of numerous defense responses, it appears that they act in a complex regulatory sub-network rather than in a linear fashion, which would be much more vulnerable to gene function loss either by pathogen-derived effectors or by mutations. In this study we employed RNA-seq, mass spectrometry and chromatin immunoprecipitation-seq to find evidence for and uncover principles and characteristics of this network. Upon flg22-treatment, one can distinguish between two sets of WRKY genes: constitutively expressed and induced WRKY genes. Prior to elicitation the induced WRKY genes appear to be maintained in a repressed state mainly by the constitutively expressed WRKY factors, which themselves appear to be regulated by non-WRKY TFs. Upon elicitation, induced WRKYs rapidly bind to induced WRKY gene promoters and by auto- and cross-regulation build up the regulatory network. Maintenance of this flg22-induced network appears highly robust as removal of three key WRKY factors can be physically and functionally compensated for by other WRKY family members.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Factores de Transcripción / Arabidopsis / Genoma de Planta / Regulación de la Expresión Génica de las Plantas / Pseudomonas syringae / Redes Reguladoras de Genes Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2018 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Factores de Transcripción / Arabidopsis / Genoma de Planta / Regulación de la Expresión Génica de las Plantas / Pseudomonas syringae / Redes Reguladoras de Genes Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2018 Tipo del documento: Article