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DNM1L, a key prognostic predictor for gastric adenocarcinoma, is involved in cell proliferation, invasion, and apoptosis.
Xu, Xiao-Wu; Yang, Xiao-Min; Zhao, Wei-Jia; Zhou, Lei; Li, De-Chun; Zheng, Yi-Hu.
Afiliación
  • Xu XW; Department of General Surgery, The Second Affiliated Hospital and Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
  • Yang XM; Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
  • Zhao WJ; Department of Pathology, Wenzhou People's Hospital, Wenzhou, Zhejiang 325000, P.R. China.
  • Zhou L; Department of General Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325002, P.R. China.
  • Li DC; Department of General Surgery, The Second Affiliated Hospital and Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
  • Zheng YH; Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
Oncol Lett ; 16(3): 3635-3641, 2018 Sep.
Article en En | MEDLINE | ID: mdl-30127972
ABSTRACT
Dynamin-1-like protein (DNM1L) encodes a member of the dynamin superfamily of GTPases. It mediates mitochondrial and peroxisomal division and is involved in the regulation of apoptosis. However, its role in gastric cancer remains unclear. MKN-45 gastric cancer cells were transfected with short hairpin RNA (shRNA) to suppress DNM1L expression. MTT, flow cytometry, and Transwell assays were used to detect the changes in cell proliferation, apoptosis, and invasion, respectively. Immunohistochemistry was used to detect DNM1L expression in gastric adenocarcinoma specimens, and the association of DNM1L expression with clinicopathological features and prognosis was analyzed. After the suppression of endogenous DNM1L expression in MKN-45 cells with shRNA, cell proliferation and invasion rates were significantly reduced, whereas apoptosis was significantly increased (all P<0.01). The expression of DNM1L was significantly higher in gastric adenocarcinoma specimens compared with that in pericarcinoma tissues (P<0.001). The expression of DNM1L increased with increasing infiltration depth, lymphatic metastasis, and higher tumor node metastasis stage (P<0.05). The expression of DNM1L associated negatively with prognosis (P<0.01). DNM1L plays a critical role in the proliferation, invasion and apoptosis of human gastric adenocarcinoma. DNM1L expression has prognostic significance for the survival of patients with gastric adenocarcinoma.
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Texto completo: 1 Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Oncol Lett Año: 2018 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Oncol Lett Año: 2018 Tipo del documento: Article