One-Pot Dual Labeling of IgG 1 and Preparation of C-to-C Fusion Proteins Through a Combination of Sortase A and Butelase 1.
Bioconjug Chem
; 29(10): 3245-3249, 2018 10 17.
Article
en En
| MEDLINE
| ID: mdl-30231608
Site-specific chemical modification of proteins can assist in the study of their function. Furthermore, these methods are essential to develop biologicals for diagnostic and therapeutic use. Standard protein engineering protocols and recombinant expression enable the production of proteins with short peptide tags recognized by enzymes capable of site-specific modification. We report here the application of two enzymes of orthogonal specificity, sortase A and butelase 1, to prepare non-natural C-to-C fusion proteins. Using these enzymes, we further demonstrate site-selective installation of different chemical moieties at two sites in a full-size antibody molecule.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
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Proteínas Recombinantes de Fusión
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Inmunoglobulina G
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Cisteína Endopeptidasas
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Aminoaciltransferasas
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Ligasas
Tipo de estudio:
Guideline
Idioma:
En
Revista:
Bioconjug Chem
Asunto de la revista:
BIOQUIMICA
Año:
2018
Tipo del documento:
Article