Improvement of extracellular secretion efficiency of Bacillus naganoensis pullulanase from recombinant Escherichia coli: Peptide fusion and cell wall modification.

Wang, Xinye; Chen, Yinqi; Nie, Yao; Xu, Yan

Wang, Xinye; Chen, Yinqi; Nie, Yao; Xu, Yan.

Afiliación

Wang X; School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
Chen Y; School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
Nie Y; School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China; Suqian Industrial Technology Research Institute of Jiangnan University, Suqian, 223814, China. Electronic address: ynie@jiangnan.edu.cn.
Xu Y; School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; The 2011 Synergetic Innovation Center of Food Safety and Nutrition,

Protein Expr Purif ; 155: 72-77, 2019 03.

Article en En | MEDLINE | ID: mdl-30248450

Asunto(s)

Bacillus/enzimología; Escherichia coli/genética; Glicósido Hidrolasas/genética; Bacillus/genética; Bacillus/metabolismo; Pared Celular/metabolismo; Clonación Molecular; Escherichia coli/metabolismo; Glicósido Hidrolasas/metabolismo; Permeabilidad; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo

Palabras clave

Amino acid tag; D,D-carboxypeptidase; Extracellular secretion; Negatively charged peptide; Pullulanase

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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Bacillus / Escherichia coli / Glicósido Hidrolasas Idioma: En Revista: Protein Expr Purif Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article

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