Reducing effects of particle adsorption to the air-water interface in cryo-EM.
Nat Methods
; 15(10): 793-795, 2018 10.
Article
en En
| MEDLINE
| ID: mdl-30250056
ABSTRACT
Most protein particles prepared in vitreous ice for single-particle cryo-electron microscopy (cryo-EM) are adsorbed to air-water or substrate-water interfaces, which can cause the particles to adopt preferred orientations. By using a rapid plunge-freezing robot and nanowire grids, we were able to reduce some of the deleterious effects of the air-water interface by decreasing the dwell time of particles in thin liquid films. We demonstrated this by using single-particle cryo-EM and cryo-electron tomography (cryo-ET) to examine hemagglutinin, insulin receptor complex, and apoferritin.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Apoferritinas
/
Receptor de Insulina
/
Agua
/
Microscopía por Crioelectrón
/
Aire
/
Hemaglutininas
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2018
Tipo del documento:
Article