The Compound Characteristics Comparison (CCC) approach: a tool for improving confidence in natural compound identification.

ABSTRACT

Compound identification is the main hurdle in LC-HRMS-based metabolomics, given the high number of 'unknown' metabolites. In recent years, numerous in silico fragmentation simulators have been developed to simplify and improve mass spectral interpretation and compound annotation. Nevertheless, expert mass spectrometry users and chemists are still needed to select the correct entry from the numerous candidates proposed by automatic tools, especially in the plant kingdom due to the huge structural diversity of natural compounds occurring in plants. In this work, we propose the use of a supervised machine learning approach to predict molecular substructures from isotopic patterns, training the model on a large database of grape metabolites. This approach, called 'Compounds Characteristics Comparison' (CCC) emulates the experience of a plant chemist who 'gains experience' from a (proof-of-principle) dataset of grape compounds. The results show that the CCC approach is able to predict with good accuracy most of the sub-structures proposed. In addition, after querying MS/MS spectra in Metfrag 2.2 and applying CCC predictions as scoring terms with real data, the CCC approach helped to give a better ranking to the correct candidates, improving users' confidence in candidate selection. Our results demonstrated that the proposed approach can complement current identification strategies based on fragmentation simulators and formula calculators, assisting compound identification. The CCC algorithm is freely available as R package (https//github.com/lucanard/CCC) which includes a seamless integration with Metfrag. The CCC package also permits uploading additional training data, which can be used to extend the proposed approach to other systems biological matrices. List of abbreviations Acidic acidic moiety; aliph aliphatic chain; AUC area under the ROC curve; bs best glycosidic structure; CCC Compounds' Characteristics Comparison; Cees Carbons estimation errors; CO Carbon to Oxygen ratio; Het Heterocyclic moiety; IMD Isotopic Mass Defect (and Pattern); LC-HRMS Liquid Chromatography - High Resolution Mass Spectrometry; md mass defect; MM Monoisotopic Mass; MS Mass Spectrometry; MSE Mean Squared Error; nC number of Carbons; NN Nitrogen; pC percentage of Carbon mass on the total mass; Pho Phosphate; PLSr Partial Least Square regression; ppm parts per million; QSRR Quantitative structure-retention relationship; RMD Relative Mass Defect; ROC Receiver Operating Characteristics; rRMD residual Relative Mass Defect; RT retention time; Sul Sulphur; UPLC-ESI-Q-TOF-MS Ultra Performance Liquid Chromatography - ElectroSpray Ionization -Quadropole - Time of Flight - Mass Spectrometry; VAT Vitis arizonica Texas.