Re-sequencing Resources to Improve Starch and Grain Quality in Rice.
Methods Mol Biol
; 1892: 201-240, 2019.
Article
en En
| MEDLINE
| ID: mdl-30397808
ABSTRACT
Next-generation sequencing can identify differences in the rice genome that explain the genetic basis of grain quality variation. Differences in rice grain quality are mainly associated with differences in the major component of the grain, starch. Association of rice quality variation with rice genome variation can be conducted at the gene or whole-genome level. Re-sequencing of specific genes or whole genomes can be used depending on the extent to which candidate genes for the traits of interest are known. Amplicon sequencing of genes involved in starch metabolism can help in targeted discovery of the molecular genetic basis of differences in starch related quality attributes. Whole-genome re-sequencing can complement this, when the genetic basis of the trait is expected to be outside the coding region of starch metabolism genes. These approaches have been used successfully to understand the rice genome at specific loci and over the whole genome.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Oryza
/
Almidón
/
Calidad de los Alimentos
/
Grano Comestible
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article