Characterization of HPV integration, viral gene expression and E6E7 alternative transcripts by RNA-Seq: A descriptive study in invasive cervical cancer.
Genomics
; 111(6): 1853-1861, 2019 12.
Article
en En
| MEDLINE
| ID: mdl-30552977
Scarce data are available on the expression of papillomavirus genome and the frequency of alternatively spliced E6E7 mRNAs in invasive cervical cancer. We carried out a comprehensive characterization of HPV expression by RNA-Seq analysis in 22 invasive cervical cancer with HPV16 or HPV18, characterizing the presence of integrated/episomal viral DNA, the integration sites in human genome and the proportion of alternative splicing products of E6 and E7 genes. The expression patterns suggested the presence of episomal and/or integrated viral DNA, with integration detected in most tumors, frequently occurring within human genes in HPV18+ and in intergenic regions in HPV16+ tumors. Alternative splicing of E6E7 transcripts showed E6*I as the most frequent isoform for both viral types, followed by E6*II and E6/E7 (unspliced) transcripts in HPV16+, and by E6/E7 in HPV18+ tumors. Previously described E6*VI and E6*V transcript isoforms for HPV16, and E6*X for HPV18, were rare or not detected.
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Base de datos:
MEDLINE
Asunto principal:
Proteínas Represoras
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Regulación Neoplásica de la Expresión Génica
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Regulación Viral de la Expresión Génica
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Neoplasias del Cuello Uterino
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Proteínas Oncogénicas Virales
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Integración Viral
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Proteínas de Unión al ADN
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Papillomavirus Humano 16
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Papillomavirus Humano 18
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Proteínas E7 de Papillomavirus
Idioma:
En
Revista:
Genomics
Asunto de la revista:
GENETICA
Año:
2019
Tipo del documento:
Article