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A novel helper qPCR system for platinum detection via Pt-DNA coordination.
Cai, Sheng; Yang, Dan; Tian, Xueke; Ye, Jiawei; Xu, Mingcheng; Abdullah Al-Maskri, Abdu Ahmed; Jung, Cheulhee; Zeng, Su.
Afiliación
  • Cai S; Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
  • Yang D; Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
  • Tian X; Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
  • Ye J; Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
  • Xu M; Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
  • Abdullah Al-Maskri AA; Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
  • Jung C; Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea. Electronic address: damo363@korea.ac.kr.
  • Zeng S; Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou, Zhejiang, 310058, China. Electronic address: zengsu@zju.edu.cn.
Anal Chim Acta ; 1050: 154-160, 2019 Mar 07.
Article en En | MEDLINE | ID: mdl-30661583
A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Platino (Metal) / ADN / Reacción en Cadena en Tiempo Real de la Polimerasa Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chim Acta Año: 2019 Tipo del documento: Article
Texto completo
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Platino (Metal) / ADN / Reacción en Cadena en Tiempo Real de la Polimerasa Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chim Acta Año: 2019 Tipo del documento: Article