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An EDS1 heterodimer signalling surface enforces timely reprogramming of immunity genes in Arabidopsis.
Bhandari, Deepak D; Lapin, Dmitry; Kracher, Barbara; von Born, Patrick; Bautor, Jaqueline; Niefind, Karsten; Parker, Jane E.
Afiliación
  • Bhandari DD; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 50829, Cologne, Germany.
  • Lapin D; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 50829, Cologne, Germany.
  • Kracher B; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 50829, Cologne, Germany.
  • von Born P; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 50829, Cologne, Germany.
  • Bautor J; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 50829, Cologne, Germany.
  • Niefind K; Department of Chemistry, Institute of Biochemistry, University of Cologne, Zuelpicher Strasse 47, 50674, Cologne, Germany.
  • Parker JE; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 50829, Cologne, Germany. parker@mpipz.mpg.de.
Nat Commun ; 10(1): 772, 2019 02 15.
Article en En | MEDLINE | ID: mdl-30770836
Plant intracellular NLR receptors recognise pathogen interference to trigger immunity but how NLRs signal is not known. Enhanced disease susceptibility1 (EDS1) heterodimers are recruited by Toll-interleukin1-receptor domain NLRs (TNLs) to transcriptionally mobilise resistance pathways. By interrogating the Arabidopsis EDS1 ɑ-helical EP-domain we identify positively charged residues lining a cavity that are essential for TNL immunity signalling, beyond heterodimer formation. Mutating a single, conserved surface arginine (R493) disables TNL immunity to an oomycete pathogen and to bacteria producing the virulence factor, coronatine. Plants expressing a weakly active EDS1R493A variant have delayed transcriptional reprogramming, with severe consequences for resistance and countering bacterial coronatine repression of early immunity genes. The same EP-domain surface is utilised by a non-TNL receptor RPS2 for bacterial immunity, indicating that the EDS1 EP-domain signals in resistance conferred by different NLR receptor types. These data provide a unique structural insight to early downstream signalling in NLR receptor immunity.
Asunto(s)

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis Tipo de estudio: Prognostic_studies Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2019 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis Tipo de estudio: Prognostic_studies Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2019 Tipo del documento: Article